SWOTein method evaluates the contribution of each residue in a protein (or protein chain) to its overall stability. The predictions are based on the three dimensional structure of the protein. Residues that contribute very unfavorably to the folding free energy are defined as stability weaknesses and colored in a red scale, whereas residues that show a highly stabilizing contribution are called stability strengths and colored in blu.

  • Press Query to submit a query to SWOTein.
  • Press Result to check on your results.
  • Press Help for additional help and information.


M. De Laet M, D. Gilis, M. Rooman, Stability strengths and weaknesses in protein structures detected by statistical potentials. Application to bovine seminal ribonuclease, Proteins 84(1):143-58 (2016).

Q. Hou, F. Pucci, F. Ancien, J.M. Kwasigroch, R. Bourgeas, M. Rooman, SWOTein: a structure-based method to predict the stability Strengths and Weaknesses in prOTEINs, Bioinformatics, btab034 (2021)